Alkylation specificities of the polyamide conjugates

Copyright information:Taken from "Unanticipated differences between α- and γ-diaminobutyric acid-linked hairpin polyamide-alkylator conjugates"Nucleic Acids Research 2006;35(1):307-316.Published online 14 Dec 2006PMCID:PMC1802559.© 2006 The Author(s) Thermal cleavage assay experiments with (), (), () and () on the 280 bp, 5′ end-labeled PCR product of pMFST2: lane 1, A reaction; lane 2, G reaction; lanes 3–13, 100, 30, 10, 3, 1 nM; 300, 100, 30, 10, 3 and 1 pM polyamide, respectively; lane 14, intact DNA. Putative major sites of alkylation on the DNA fragment are indicated by arrows on the sequences adjacent to each gel.