MOESM4 of Organotypic slice culture model demonstrates inter-neuronal spreading of alpha-synuclein aggregates

Additional file 4: Figure S4: Region- and time-dependent development of various α-syn inclusion patterns. a 7 to 10 dpi of PFFs, the pS129-positive α-syn aggregates (11A5) present as filamentous structures that surround the DAPI-stained nuclei. Scale bars: 10 μm. b 14 dpi of PFFs, the pS129-positive aggregates at DG are still filamentous, while inclusions at CA regions, mainly at CA1, present as spherical, denser cytoplasmic inclusions resembling Lewy bodies. Scale bars: 10 μm. Representative images from minimum 13 slices/3 separate experiments per time point.