MOESM4 of Role of membrane compartment occupied by Can1 (MCC) and eisosome subdomains in plant pathogenicity of the necrotrophic fungus Alternaria brassicicola

Additional file 4: Figure S3: Generation of ∆abpil1a, ∆abpil1b, ∆abslm1, and ∆abnce102 by homologous recombination. a Schematic representation of a targeted locus in WT and in mutant strains after integration of the replacement construct containing the hygromycin B (Hph gene) or the nourseothricin (Nat gene) resistance cassette and flanking sequences. Primers used for PCR screening of mutants are indicated. b Gel electrophoresis of PCR products obtained from template DNA of the WT, ∆abpil1a, ∆abpil1b, ∆abslm1, and ∆abnce102 strains with the indicated primer pairs. Molecular sizes (kb) were estimated based on a 1 kb ladder (lane L2, New England Biolabs) or SmartLadder (Lane L1, Eurogentec). ITS1/4 primers were used as a positive control for PCR.