MOESM6 of The Study of Enhanced High-Intensity Focused Ultrasound Therapy by Sonodynamic N2O Microbubbles

Additional file 6: Figure S6. In vitro ultrasound imaging performance. Fifteen grams of agarose and 500 mL of deionized (DI) water were mixed at a concentration of 3%. The mixture was repeatedly heated until the agarose dissolved. Then, after cooling, the agar gel was prepared as an in vitro ultrasound imaging model. The N2O-mbs (200 μL, 1 × 105 bubbles/mL) PBS and C3F8-mbs (200 μL, 1 × 105 bubbles/mL) PBS solutions were added to the gel model. Ultrasound imaging of the microbubbles was performed on an ultrasound system (Esaote, Italy). Finally, the echo intensities of regions of interest (ROI) were were quantitatively measured by ultrasound imaging software. The in vitro N2O-mb and C3F8-mb ultrasound imaging performance analysis is shown in Figure S6. The echo intensity of the B-mode ultrasound image within the ROI was calculated by ultrasound analysis software. The target sound intensity between the N2O-mbs group and the C3F8-mbs group was not significantly different (p > 0.05).