MOESM3 of The Study of Enhanced High-Intensity Focused Ultrasound Therapy by Sonodynamic N2O Microbubbles

Additional file 3: Figure S3. Quantitative analysis of 1O2 generation of N2O irradiated by LIFU. (The data were shown as mean ± SD, n = 5 per group, *p < 0.05). In aqueous solution, the difference between the N2O treated with ultrasound (LIFU-N2O group) and the N2O group was not statistically significant (p > 0.05). Compared with the control group, fluorescence intensity increased only in the LIFU group and LIFU-N2O group. And the addition of N2O did not increase the generation of oxygen free radicals in the LIFU-N2O group (p > 0.05). In the LIFU group and LIFU-N2O group, a small amount of oxygen free radicals was generated, possibly only because of the stimulation of ultrasound.