MOESM2 of Fibrosis independent atrial fibrillation in older patients is driven by substrate leukocyte infiltration: diagnostic and prognostic implications to patients undergoing cardiac surgery
Additional file 2: Figure S2. Macrophage isolation purity. (A) Dot plot representing the variability of obtainable tissue weight. (B) PBMC isolation comparison between two different techniques. Technique 1: incubation of tissue with collagenase for 30 min. Technique 2: incubation of tissue with coll...
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| creator | Aguiar, Christie Gawdat, Kareem Legere, Stephanie Marshall, Jean Hassan, Ansar Kienesberger, Petra Pulinilkunnil, Thomas Castonguay, Mathieu Brunt, Keith Jean-Francois Legare |
| description | Additional file 2: Figure S2. Macrophage isolation purity. (A) Dot plot representing the variability of obtainable tissue weight. (B) PBMC isolation comparison between two different techniques. Technique 1: incubation of tissue with collagenase for 30 min. Technique 2: incubation of tissue with collagenase + dispase for 45 min. Technique 2 yielded a higher cell isolation that was significantly different from technique 1 (P = 0.02). |
| doi_str_mv | 10.6084/m9.figshare.11352848 |
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Macrophage isolation purity. (A) Dot plot representing the variability of obtainable tissue weight. (B) PBMC isolation comparison between two different techniques. Technique 1: incubation of tissue with collagenase for 30 min. Technique 2: incubation of tissue with collagenase + dispase for 45 min. 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Macrophage isolation purity. (A) Dot plot representing the variability of obtainable tissue weight. (B) PBMC isolation comparison between two different techniques. Technique 1: incubation of tissue with collagenase for 30 min. Technique 2: incubation of tissue with collagenase + dispase for 45 min. Technique 2 yielded a higher cell isolation that was significantly different from technique 1 (P = 0.02).</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.11352848</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Biochemistry Biological Sciences not elsewhere classified Biophysics Cell Biology Computational Biology Developmental Biology Environmental Sciences not elsewhere classified FOS: Biological sciences FOS: Clinical medicine FOS: Earth and related environmental sciences FOS: Health sciences FOS: Physical sciences Immunology Infectious Diseases Marine Biology Molecular Biology Physical Sciences not elsewhere classified Physiology Virology |
| title | MOESM2 of Fibrosis independent atrial fibrillation in older patients is driven by substrate leukocyte infiltration: diagnostic and prognostic implications to patients undergoing cardiac surgery |
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