MOESM2 of Fibrosis independent atrial fibrillation in older patients is driven by substrate leukocyte infiltration: diagnostic and prognostic implications to patients undergoing cardiac surgery

Additional file 2: Figure S2. Macrophage isolation purity. (A) Dot plot representing the variability of obtainable tissue weight. (B) PBMC isolation comparison between two different techniques. Technique 1: incubation of tissue with collagenase for 30 min. Technique 2: incubation of tissue with collagenase + dispase for 45 min. Technique 2 yielded a higher cell isolation that was significantly different from technique 1 (P = 0.02).