MOESM3 of Putative stem cells in the hemolymph and in the intestinal submucosa of the solitary ascidian Styela plicata

Additional file 3: Figure S2. Evaluation of PIWI antibody cross-reactivity. (A) Alignment of PIWI proteins in the fly Drosophila melanogaster (Dromel), the annelid Pristina leidyi (Prilei), and ascidians: Styela plicata (Stypli), Botrylloides leachi (Botlea), Botryllus primigenus (Botpri), Botryllus schlosseri (Botsch), and Ciona robusta (Ciorob). Conserved PAZ and PIWI domains are shown below the Drosophila melanogaster PIWI sequence (Dromel_piwi_varA). The sequence region used to generate the epitope for the commercially available antibody used in this study is also shown overlapping the PAZ domain (582–689 aa). Color codes used to represent sequence conservation (Blosum62 score matrix for similarities): white (