Secondary screening of combinatorial mutants cleaving COMB2

Copyright information:Taken from "A combinatorial approach to create artificial homing endonucleases cleaving chosen sequences"Nucleic Acids Research 2006;34(22):e149-e149.Published online 27 Nov 2006PMCID:PMC1702487.© 2006 The Author(s). Upper panel: map of the mutants feature on the following panels. As described in text, combinatorial mutants are named with a eight letter code, after residues at positions 28, 30, 33, 38, 40, 44, 68 and 70 and parental controls with a five letter or three letter code, after residues at positions 28, 30, 33, 38 and 40 or 44, 68 and 70. Mutants are screened in yeast against COMB2 and 10TGC and 5GAC, the two parental targets.