Genotyping of strains evaluated by VNTR

Copyright information:Taken from "Exploring glycopeptide-resistance in : a combined proteomics and transcriptomics approach for the identification of resistance-related markers"BMC Genomics 2006;7():296-296.Published online 22 Nov 2006PMCID:PMC1687195. Based on the limit of clonality of the assay (0.1 unit of divergence) [77], our collection contains at least 4 unrelated genetic backgrounds (noted A-D). Control strains whose genome has been sequenced have been simultaneously analyzed. Gene expression of identified markers on a collection of strains showing altered susceptibility to glycopeptides. Relative expression of 6 gene markers found up-regulated in the resistant strain 14-4 compared to the two sensitive strains. The same markers were also quantified in a collection of strains from the NARSA, selected for their resistance to glycopeptide. Values are mean + standard deviation of triplicate measurements from 2 independent experiments. Increase expression of selected markers, linked to glycopeptide resistance is independent on the background. The complete raw microarray dataset has been posted on the Gene Expression Omnibus database [78], accession number GSE5188. All additional files are freely accessible [79].