Northern blot analysis of total RNA from different varieties of rice

Copyright information:Taken from "An ABRE-binding factor, OSBZ8, is highly expressed in salt tolerant cultivars than in salt sensitive cultivars of indica rice"BMC Plant Biology 2006;6():18-18.Published online 30 Aug 2006PMCID:PMC1574319. A. Blot with equal amount of total RNA isolated from roots of Pokkali plants probed with [P]-(encoding highly conserved basic domain), showed hybridization with two transcripts of size 2.2 kb and 1.3 kb, both of which appeared after treatment of plants either with 200 mM NaCl (lane 2) or with 100 μM ABA (lane 3) or with NaCl and cycloheximide (Ch, lane 4), together. B. Physical map of the entire ORF for OSBZ8 showing the positions of the primers used in the amplification of full length or . C. Northern blot with total RNA from roots (R) and lamina (L) of M-I-48 and Nonabokra probed with the unique region of OSBZ8. Transcript abundance is shown both in control and treatment with 200 mM NaCl (lane 1, 2, 3 and 4 in M-I-48; and lane 5, 6, 7 and 8 in Nonabokra). D. Northern blot with total RNA from lamina of M-I-48 (lane 1 and 2), and Nonabokra (lane 3 and 4), probed with full length. Histogram drawn from the values obtained by the densitometric scanning of each lane is shown under each autoradiogram. Mean values ± SD of three independent experiments are shown.