MOESM4 of Effects of cigarette smoke on barrier function and tight junction proteins in the bronchial epithelium: protective role of cathelicidin LL-37

Additional file 4: CSE-induced production of LL-37 in culture medium. Calu-3 cells were exposed to 10% CSE and then cell culture supernatants in apical and basal chambers were collected at 8, 18, 24 h after CSE exposure. The concentration of LL-37 in culture supernatants were measured by ELISA. All results are representative of at least two independent experiments. Data represent means ± SEM (n = 5 per group). *p