CIL:13161, Mus musculus, fibroblast. In Cell Image Library

CIL:13161, Mus musculus, fibroblast. In Cell Image Library

Transition from 1D to 2D migration: regulation of cellular phenotype by ECM topography. Formation of multiaxial lamellae and cell spreading during the transition from 1D to 2D migration. 2D matrices were constructed by uniform coating with extracellular matrix (ECM). 1D surfaces were made by using a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Doyle, Andrew D., Wang, Francis W., Matsumoto, Kazue, Yamada, Kenneth M.
Format: Dataset
Sprache:eng
Schlagworte:
cell
cell migration
fibroblast
Mus musculus
NIH/3T3
substrate-dependent cell migration, cell attachment to substrate
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Beschreibung
Zusammenfassung:Transition from 1D to 2D migration: regulation of cellular phenotype by ECM topography. Formation of multiaxial lamellae and cell spreading during the transition from 1D to 2D migration. 2D matrices were constructed by uniform coating with extracellular matrix (ECM). 1D surfaces were made by using a 2 photon confocal microscope to pattern a polyvinyl alchohol (PVA) coated coverslip. Following gluteraldehyde quenching, ECM proteins were absorbed onto the PVA surface. Movie is video 6 from JCB 2009, 184:481-490
DOI:10.6075/j0m0445f