Comparative Chloroplast Genome Analyses Among Three Sympatric Species of Atraphaxis

DNA was extracted using an improved CTAB method and stored in a refrigerator at -20 ° C. After the DNA samples pass the test, the Covaris ultrasonic crusher is used to randomly break the qualified DNA samples; The preparation of the library was completed through multiple steps such as end repair, addition of A-tail, addition of sequencing connectors, purification, and PCR amplification; After quality inspection, the Illumina Hiseq 2000 platform of Beijing, China was used to sequence the entire genomes of three species, namely Polygonum spinosum, Polygonum frontalis, and Polygonum slender. DNA was extracted using an improved CTAB method and stored in a refrigerator at -20 ° C. After the DNA samples pass the test, the Covaris ultrasonic crusher is used to randomly break the qualified DNA samples; The preparation of the library was completed through multiple steps such as end repair, addition of A-tail, addition of sequencing connectors, purification, and PCR amplification; After quality inspection, the Illumina