The first discovery of severe fever with thrombocytopenia virus in the center of metropolitan Beijing, China

All data were collected from H. longicornis ticks that were sourced from Beijing in 2023. To gather data on the ticks, we utilized full-length mitochondrial sequencing. Specifically, one leg from each tick was removed for analysis, and tick DNA was extracted using the MightyPrep reagent for DNA Kit (Takara, Japan), following the manufacturer's instructions. The extracted mitochondrial DNA was then sequenced using next-generation sequencing technology.For viral data collection, total RNAs were extracted from tick homogenates and hedgehog blood cells using either TRIzol reagent (Thermo Fisher Scientific, USA) or the RNeasy kit (Qiagen, Germany), in accordance with the respective manufacturer's protocols. The RNA samples were then reverse transcribed using a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan). Following reverse transcription, the target viral fragments were amplified using the nested PCR method. The resulting PCR products were then sequenced by the Sanger method for further analysis. All data were collected from H. longicornis ticks that were sourced from Beijing in 2023. To gather data on the ticks, we utilized full-length mitochondrial sequencing. Specifically, one leg from each tick was removed for analysis, and tick DNA was extracted using the MightyPrep reagent for DNA Kit (Takara, Japan), following the manufacturer's instructions. The extracted mitochondrial DNA was then sequenced using next-generation sequencing technology.For viral data collection, total RNAs were extracted from tick homogenates and hedgehog blood cells using either TRIzol reagent (Thermo Fisher Scientific, USA) or the RNeasy kit (Qiagen, Germany), in accordance with the respective manufacturer's protocols. The RNA samples were then reverse transcribed using a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan). Following reverse transcription, the target viral fragments were amplified using the nested PCR method. The resulting PCR products were then sequenced by the Sanger method for further analysis.