Supporting data for "A Database of Restriction Maps to Expand the Utility of Bacterial Artificial Chromosomes"
While Bacterial Artificial Chromosomes were once a key resource for the genomic community, they have been obviated, for sequencing purposes, by long-read technologies. Such libraries may now serve as a valuable resource for manipulating and assembling large genomic constructs. To enhance accessibili...
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Zusammenfassung: | While Bacterial Artificial Chromosomes were once a key resource for the genomic community, they have been obviated, for sequencing purposes, by long-read technologies. Such libraries may now serve as a valuable resource for manipulating and assembling large genomic constructs. To enhance accessibility and comparison, we have developed a BAC restriction map database. Using information available from the National Center for Biotechnology Information’s cloneDB ftp site, a database containing the restriction maps for both uniquely placed and insert-sequenced BACs from 11 libraries covering available restriction enzyme recognition sequences was constructed. Along with the database, a set of python functions are available for reconstructing the database and more easily accessing information within. This data is valuable for researchers simply using BACs, as well as working with larger sections of the genome in terms of synthetic genes, large-scale editing, and mapping. |
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DOI: | 10.5524/102451 |