Fig. 7 in Mutagenesis of a Lotus japonicus GSK3β/Shaggy-like kinase reveals functionally conserved regulatory residues

Fig. 7. Appearance of whole plants and nodules of L. japonicus hairy root lines transformed with the different LjSK1 variants. A, F) Control (empty vector); B, G) native LjSK1; C, H) LjSK1 K167A; D, I) LjSK1 Y298A; E, J) LjSK190-467. A-E) Representative whole plants with regenerated hairy roots. F-J) Images of nodules on regenerated hairy roots taken in bright-field (top panels) and with an RFP filter to visualize rhizobium colonisation (bottom panels). Scale bars in A-E are 5 cm and in F-G 2 mm.