Fig. 2 in Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 Co-cultures using thermal desorption - Comprehensive two-dimensional gas chromatography - Time-of-flight mass spectrometry (TD-GC×GC-TOFMS)

Fig. 2. Workflow for sample preparation and injection. Culture samples were filtered and dried (A–B). Dried filter papers were placed in clean vials (C) and then resuspended in methanol (D) before being extracted with Chloroform (E). Water was added (F) and subsequently, the chloroform layer was aliquotted into GC vials (G) for further sample preparation. Extracts were dried (H) and then derivatized using a two-step methoximation/silylation process to yield derivatized extracts (I). 9-μL aliquots of derivatized extracts were automatically transferred to microvial inserts in thermal desorption tubes for injection (J) using an initial solvent vent step to remove excess solvent and derivatisation reagents (K), followed by thermal desorption to a cooled PTV inlet and subsequent splitless injection to the GC × GC-TOFMS system. Non-volatile residues from the extracts remained in the microvial insert for subsequent disposal (L). See text for details.