Fig. 4 in Rapid screening of glycosyltransferases in plants using a linear DNA expression template based cell-free transcription-translation system

Fig. 4. UPLC-MS analysis of isoquercitrin converted from quercetin by ArUGTs using heterologous expression. (A) The SDS-PAGE gel of heterologous expression of ArUGTs AR14572, AR11662, and AR43718 protein. The theoretical molecular weights of AR14572 protein, AR11662 protein, and AR43718 protein are 80.1 kDa, 79.8 kDa, and 80.4 kDa, respectively. (B) The chromatogram of isoquercitrin standard. (C–E) Chromatograms of products from the catalysis of quercetin by different ArUGTs using heterologous expression: (C) AR14572, (D) AR11662, and (E) AR43718, see Supplementary Fig. S6 for the duplicate group. The y axis indicates ion signal abundance relative to the highest signal in each chromatogram (%). The numbers in the top right of each chromatogram (for instance 4.56e6 in [B]) indicate the ion counts represented by "100" relative abundance. The retention time of the isoquercitrin standard is 3.93 min, and the red arrow in each figure indicates the retention time of the product isoquercitrin. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)