Fig. 5 in Multivariate analysis of chemical and genetic diversity of wild Humulus lupulus L. (hop) collected in situ in northern France

Fig. 5. Identification of population genetic structure of the 63 accessions of Humulus lupulus L. sampled in Northern France (Hauts-de-France region) using 11 microsatellites. A. Bar plot showing the distribution of individual assignations estimated for K = 2 and K = 6 clusters, from Bayesian inference cluster analysis performed with the 53 Humulus lupulus haplotypes sampled from the 14 locations (from A to K). Each vertical line represents an individual and the length of each colored line corresponds to the membership coefficient (scale at the left of the bar plot) for each cluster. Individuals are grouped according to their sampling locations. B. Frequencies of the 6 clusters (represented by colors) within each sampled location. Colors are same than on Fig. 5A. C. Principle Component Analysis (PCoA) based on genetic distances between each accession. Individuals were colored according to their sample site collection. D. Dendrogram underlying genetic clustering of the 63 hop accessions, including 10 commercial varieties (samples 1 to 10), 3 heirloom varieties (samples 11 to 13) and 50 wild sampled from 11 geographical locations (cf Table 1). 1: Nugget, 2: Strisselspalt, 3: Golding, 4: Challenger, 5: Brewers Gold, 6: Cascade, 7: Magnum, 8: Northern Brewer, 9: Target, 10: Fuggle, 11: Groene Bel, 12: Star, 13: Coigneau, Location A: 14 to 18; Location B: 19 to 23; Location C: 24 to 28; Location D: 29 to 32; Location E: 33 and 34; Location F: 34 and 35; Location G: 37 and 38; Location H: 39 to 42; Location I: 43 to 52; Location J: 53 to 58; Location K: 59 to 63. The tree was constructed using the unweighted neighbor-joining method based on genetic dissimilarity among the haplotypes according to microsatellite markers. Each branch corresponds to a hop genotype and the colors of branches indicate locations from which the genotypes were sampled. The color code is the same as the one on Fig. 5C.