Supplemental Figure S1. Variation of the Met plasma concentration (μmol/L) over 6 time points in the experiment for each treatment group (CTRL, MetLys, MetLysHis)

Supplemental Figure S1. Variation of the Met plasma concentration (μmol/L) over 6 time points in the experiment for each treatment group (CTRL, MetLys, MetLysHis). Day 1 is the reference, d18 and d29 are in the depletion period, d54 and d79 in the RP-AA period and d113 in the cross-back period. Day 29, d79 and d113 also represent the last d of each period. During the whole experiment all treatment groups received a low protein diet (CTRL). The cows in the MetLys group received rumen-protected (RP) Met (Excential Rumenpass MET, Orffa Additives) and RP-Lys (AjiPro-L, Ajinomoto H&N) during the RP-AA period. The cows in the MetLysHis group received RP-Met, RP-Lys and RP-His (experimental RP-His product, Ajinomoto Co.) during the RP-AA period. Significant differences (P ≤ 0.05) between groups within a d are indicated by different letters as determined by Tukey’s test.