RT-qPCR dCT values of genes analyzed in colon, small intestine, liver and colon organoids
RT-qPCR dCt values of gene expression from colon, small intestine and liver tissues from germ free mice monocolonized with E. coli expression bile salt hydrolase activity. Mice monocolonized with non-BSH expressing E. coli, non-monocolonized and conventionally-raised mice were used as controls, and...
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| creator | Núñez-Sánchez, María A. Herisson, Florence M. García-González, Natalia Rossini, Valerio Pinhiero, Jorge Daly, Jack Bustamante-Garrido, Milán Hueston, Cara M. Patel, Shriram Canela, Nuria Herrero, Pol Claesson, Marcus Melgar, Silvia Nally, Ken Caplice, Noel M. Gahan, Cormac G.M. |
| description | RT-qPCR dCt values of gene expression from colon, small intestine and liver tissues from germ free mice monocolonized with E. coli expression bile salt hydrolase activity. Mice monocolonized with non-BSH expressing E. coli, non-monocolonized and conventionally-raised mice were used as controls, and RT-qpCR dCT values of colon organoids incubated with a mix of bile acids, LPS or a mix of bile acids + LPS. Values are normalized to the expression levels of ß-actin |
| doi_str_mv | 10.5281/zenodo.6598103 |
| format | Dataset |
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| title | RT-qPCR dCT values of genes analyzed in colon, small intestine, liver and colon organoids |
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