RT-qPCR dCT values of genes analyzed in colon, small intestine, liver and colon organoids

RT-qPCR dCT values of genes analyzed in colon, small intestine, liver and colon organoids

RT-qPCR dCt values of gene expression from colon, small intestine and liver tissues from germ free mice monocolonized with E. coli expression bile salt hydrolase activity. Mice monocolonized with non-BSH expressing E. coli, non-monocolonized and conventionally-raised mice were used as controls, and...

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Hauptverfasser: Núñez-Sánchez, María A., Herisson, Florence M., García-González, Natalia, Rossini, Valerio, Pinhiero, Jorge, Daly, Jack, Bustamante-Garrido, Milán, Hueston, Cara M., Patel, Shriram, Canela, Nuria, Herrero, Pol, Claesson, Marcus, Melgar, Silvia, Nally, Ken, Caplice, Noel M., Gahan, Cormac G.M.
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Sprache:eng
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Zusammenfassung:RT-qPCR dCt values of gene expression from colon, small intestine and liver tissues from germ free mice monocolonized with E. coli expression bile salt hydrolase activity. Mice monocolonized with non-BSH expressing E. coli, non-monocolonized and conventionally-raised mice were used as controls, and RT-qpCR dCT values of colon organoids incubated with a mix of bile acids, LPS or a mix of bile acids + LPS. Values are normalized to the expression levels of ß-actin
DOI:10.5281/zenodo.6598103