Gold Nanoparticles Synthesized in the Presence of Peptides - UV-Vis Spectra, Fluorescence, USAXS, Electron Microscopy; pre-publication version

Content Summary: Data from experiments in which gold nanoparticles were synthesized in the presence of peptides using a liquid-handling robot. Samples were analyzed using UV-Vis spectroscopy, fluorescence emission, USAXS, TEM, and SEM. Notebooks for loading and plotting data Code for synthesizing samples using an OT2 Opentrons liquid-handling robot. README: /Data Contains all UV-Vis, electron microscopy, fluorescence, and SAXS data for gold nanoparticles synthesized in the presence of peptides and HEPES. /Data/2021_12_30_Prepared_UV_Vis_Data The primary portion of the experimental dataset. UV-Vis spectroscopy data collected on a Biotek Epoch 2 microplate spectrophotometer 24 hours after samples were synthesized using a liquid handling robot (Opentrons OT2). The 4x4x4_SI.csv file is the compilation of all sample information: Concentrations (M) of peptide, HAuCl4, and HEPES UID – unique ID based on date of synthesis, sample position, and peptide which was used to synthesize the sample. Peptide names: Z2: RMRMKMK; MZ2: myristoylated - RMRMKMK; MZ2R: myristoylated - KMKMRMR; PZ2: palmitoylated – RMRMKMK; Z2M6I: RMRMKIK; Z2M246I: RIRIKIK; AG3: AYSSGAPPMPPF. Each sample’s UID is a key to match with UV-Vis measurement result stored in the {UID}.txt files. Each of these files contains the wavelength, absorbance, and absorbance after subtraction of a water measurement. /Data/ElectronMicroscopy Scanning electron microscopy and transmission electron microscopy results of gold nanoparticles formed from the reduction of HAuCl4 in the presence or absence of different peptides. Fig A, B, C, D, E/F were prepared in the presence of Z2, Z2M6I, Z2M246I, no peptide, and MZ2R, respectively. /Data/Fluorescence Pyrene fluorescence data collected in the presence of different concentrations of lipidated peptides (MZ2, MZ2R, and PZ2) for estimation of the peptide critical micelle concentration. /Data/SAXS SAXS data of a high concentration of MZ2 which was fit using a cylindrical model form factor. The evaluated model is also shared in this directory. /Data/USAXS Similarly to the UV-Vis data directory, the USAXS_SI.csv file contains sample information for all of the USAXS measurements. The dsm_rg.csv file contains the output of AUTORG evaluated on the desmeared data after subtraction of a flat background at high-q. /DSM_Nexus, DSM_sub_AUTORG, and SMR_Nexus contain the desmeared, desmeared with background subtraction, and smeared versions of the USAXS data, respectively. /Notebooks N