Ultrafast imaging recordings from the axon initial segment of neocortical layer-5 pyramidal neurons

This dataset contains imaging and whole-cell electrophysiological recordings from neocortical layer-5 pyramidal neuron from brain slices of the mouse. Electrophysiological recordings (at 20 kHz) are from the soma. Imaging data (10 kHz) are from lines along the axon initial segment (distal>proximal) with 500 nm pixel resolution. These correspond to: Sodium imaging (Figures 1 and S6). Voltage imaging (Figures 2,4,5,S4,S7) Calcium imaging (Figures 3,S3,S8). This dataset is used in the paper available online: Filipis L, Blömer LA, Montnach J, De Waard M, Canepari M. Nav1.2 and BK channels interaction shapes the action potential in the axon initial segment. bioRxiv, 2022. doi: 10.1101/2022.04.12.488116.