Assessment of morphology and health of primary neuron and astrocyte population

Supplementary Figure 1. Assessment of morphology and health of primary neuron and astrocyte population. Primary cell culture examples on poly-D-lysine treated glass coverslips. Neuronal and astrocyte populations appear large and healthy. Neurons form clusters and extend processes, whereas astrocytes have expanded and covered the entire surface. Left image: 21 DIV. Right image: 7 DIV. For both images green channel is GFAP (astrocytes), red channel is TUJ1 (neurons) and blue channel is DAPI (nuclei). Scale bar is 100 μm.