ImageInLife20170922IP01_Sipka_Macrophage Recruitment to the Wound

A tail of 3dpf transgenic zebrafish larvae (tg(mpeg1:Gal4/UAS:Kaede)) was imaged from 1h to 6h after the tail fin amputation by ANDOR CSU-W1 confocal spinning disk on an inverted NIKON microscope (Ti Eclipse) with ANDOR Neo sCMOS camera (20x air/NA 0.75 objective, mosaic imaging 6x1 with 10% overlap between windows). Laser excitation/emission wavelength: 488/521 nm. Time step is 4min (75 repeats). Z-stack is 132μm with 4μm step (33 steps). Maximum projection is shown as well. Pixel size is 0.326μm. Macrophages expressing green fluorescent protein Kaede in the cytoplasm are moving toward the wound made at the tail fin.