Assembled "juvenile head region" transcriptome from the hagfish Eptatretus burgeri (NCBI GenBank Accession: SRX2541845)

1) The raw reads were dwonloaded from NCBI GenBank (SRA run accession: (SRR5234495) with sratoolkit. 2) The assembly was performed with Trinity with the folllowing parameters: Trinity --seqType fq --max_memory 100G --left eb-hf-head-region-SRR5234495/eb-hf-head-region-SRR5234495_1.fastq --right eb-hf-head-region-SRR5234495/eb-hf-head-region-SRR5234495_1.fastq --CPU 10 --output trinity-transcriptome-eb/ 3) ORFs were predeicted with TransDecoder with the following settings: TransDecoder.LongOrfs -t transcriptome-eb-hf-head-region.fasta TransDecoder.Predict -t transcriptome-eb-hf-head-region.fasta