Data archive for: Resting cells of Skeletonema marinoi assimilate organic compounds and respire by dissimilatory nitrate reduction to ammonium in dark, anoxic conditions

Data archive for: “Resting cells of Skeletonema marinoi assimilate organic compounds and respire by dissimilatory nitrate reduction to ammonium in dark, anoxic conditions” https://doi.org/10.1111/1462-2920.16625   Dataset of single cell assimilation of organic/inorganic C/N by resting cells of the m...

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Hauptverfasser: Stenow, Rickard, Robertson, Elizabeth K., Kourtchenko, Olga, Whitehouse, Martin J., Pinder, Matthew I. M., Benvenuto, Giovanna, Töpel, Mats, Anna, Godhe, Ploug, Helle
Format: Dataset
Sprache:eng
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Zusammenfassung:Data archive for: “Resting cells of Skeletonema marinoi assimilate organic compounds and respire by dissimilatory nitrate reduction to ammonium in dark, anoxic conditions” https://doi.org/10.1111/1462-2920.16625   Dataset of single cell assimilation of organic/inorganic C/N by resting cells of the marine diatom Skeletonema marinoi captured using secondary ion mass spectrometry (SIMS) and stable isotopic tracers. The dataset also contains POC/PON changes over time during dormancy, DNRA (15N-NH4+ production), denitrification (15N-N2 production) and a germination assay to determine survival rate, most probable number analysis (MPN).   Two strains (GF04 and R05) were incubated in dark and anoxic conditions in two different incubation experiments. Incubation 1: Diatoms treated with antibiotics before entering dormancy compared to a control not treated with antibiotics then given 15N-NO3- in dark anoxic conditions. Incubation 2: Diatoms treated with antibiotics given, 15N & 13C urea, 15N & 13C urea + 14N-NO3-, 13C-acetate, 13C-acetate + 15N-NO3-, or 15N-NO3-. See the main manuscript for a extensive experimental setup.   Each file is uploaded as both a .CSV and .XLSX, so that you can choose which you prefer. DNRA_and_denitrification.csv/xlsx: DRNA and denitrification depending on volume (Incubation 1) DNRA_per_cell.csv/xlsx: DNRA per cell (Incubation 1 & 2) MPN_data.csv/xlsx: Most probable number analysis (Incubation 1 & 2) POC_PON.csv/xlsx: POC and PON per cell and volume (Incubation 1 & 2) SIMS_data.csv/xlsx: SIMS data (Incubation 1 & 2)    
DOI:10.5281/zenodo.10725962