Data from: Trophic position and niche overlap of an Asian weatherfish (Misgurnus bipartitus), western tubenose goby (Proterorhinus semilunaris), and native benthic fish species

The dataset belonging to the paper "Trophic position and niche overlap of an Asian weatherfish (Misgurnus bipartitus), western tubenose goby (Proterorhinus semilunaris), and native benthic fish species" published in Aquatic Invasions (paper in press; doi link will be added later), is provided here. The dataset consists of δ13C and δ15N (‰) stable isotope ratios of taxa of fish, macroinvertebrates, plants, alga, and soil. If applicable, the length of the taxon was included. Below, methodological information is provided on the study site, the sampling process, the sampling preparation, and the stable isotope analysis. For references, see the published paper in Aquatic Invasions. Study site The study site concerned a section of the lowland brook Tungelroysebeek (51°14.38'N, 005°52.086'E – 51°14.26'N, 005°47.77'E) near the village Tungelroy in the Province of Limburg, the Netherlands. This brook of 35 km length has several tributaries before discharging into the River Meuse. Over most of its course, the brook was meandering and had a well-developed riparian and hydrophyte vegetation. During sampling the mean water temperature was 16.0 °C, conductivity 664 µS/cm, pH 7.3, water velocity 0.2 m/s, depth 70 cm, and Secchi depth 60 cm. The brook width ranged between 5-8 m and its bed substrate predominantly consisted of sand. Sampling Samples of fish, macroinvertebrates, macrophytes, dead organic material, and bottom soil substrate were collected in October 2019. The samples were collected in the stretch of the brook that is denoted in Fig. 1 of the paper. Fish were caught using handheld electrofishing equipment (Bretschneider EFGI 650). After catching, the fish were euthanized using a neutralized benzocaine solution of 100 mg l-1. Macroinvertebrates were collected using 70x55 cm dip nets with a mesh size of 1 mm. Helophytes, floating-leaved, and submerged aquatic macrophytes were collected by hand. Soil samples were collected by means of a hollow soil sampling tube with a diameter of 5 cm. A Nikon SMZ800 stereo microscope with a 10-63 magnification was used for the identification of small macroinvertebrates. Sample preparation All samples were transported to the laboratory and stored separately at -18° C until preparation. To obtain muscle samples a piece of 0.5-1 cm of the dorsal tissue of each fish was dissected. Muscle tissue samples were dissected from fish, crayfish, and unionid mussels. Other macroinvertebrates were stored alive for two days at 5 °C to