Data from: Treated like dirt: Robust forensic and ecological inferences from soil eDNA after challenging sample storage
We investigated the effect of storage duration and conditions on the assessment of the soil biota with eDNA metabarcoding. We extracted eDNA from freshly collected soil samples and again from the same samples after storage under contrasting temperature conditions and contrasting exposure (open/close...
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Sprache: | eng |
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Zusammenfassung: | We investigated the effect of storage duration and conditions on the
assessment of the soil biota with eDNA metabarcoding. We extracted eDNA
from freshly collected soil samples and again from the same samples after
storage under contrasting temperature conditions and contrasting exposure
(open/closed tubes). We used four different primer sets targeting
bacteria, fungi, protists (cercozoans), and general eukaryotes. We
quantified differences in richness, evenness, and community composition.
Subsequently, we tested whether we could correctly infer habitat type and
original sample identity after storage using a large reference dataset.
This repository contains the un-demultiplexed fastq sequences. |
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DOI: | 10.5061/dryad.k0p2ngfbs |