Data from: Quantification and statistical analysis methods for vessel wall components from stained images with Masson's trichrome
Purpose: To develop a digital image processing method to quantify structural components (smooth muscle fibers and extracellular matrix) in the vessel wall stained with Masson’s trichrome, and a statistical method suitable for small sample sizes to analyze the results previously obtained. Methods: Th...
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Zusammenfassung: | Purpose: To develop a digital image processing method to quantify
structural components (smooth muscle fibers and extracellular matrix) in
the vessel wall stained with Masson’s trichrome, and a statistical method
suitable for small sample sizes to analyze the results previously
obtained. Methods: The quantification method comprises two stages. The
pre-processing stage improves tissue image appearance and the vessel wall
area is delimited. In the feature extraction stage, the vessel wall
components are segmented by grouping pixels with a similar color. The area
of each component is calculated by normalizing the number of pixels of
each group by the vessel wall area. Statistical analyses are implemented
by permutation tests, based on resampling without replacement from the set
of the observed data to obtain a sampling distribution of an estimator.
The implementation can be parallelized on a multicore machine to reduce
execution time. Results: The methods have been tested on 48 vessel wall
samples of the internal saphenous vein stained with Masson’s trichrome.
The results show that the segmented areas are consistent with the
perception of a team of doctors and demonstrate good correlation between
the expert judgments and the measured parameters for evaluating vessel
wall changes. Conclusion: The proposed methodology offers a powerful tool
to quantify some components of the vessel wall. It is more objective,
sensitive and accurate than the biochemical and qualitative methods
traditionally used. The permutation tests are suitable statistical
techniques to analyze the numerical measurements obtained when the
underlying assumptions of the other statistical techniques are not met. |
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DOI: | 10.5061/dryad.fh0m2 |