Assembled and extracted single copy orthologue (SCO) sequences from Rosa canina genome, diploid Rosa species, and sect. Caninae pollen
This dataset was created to analyze nuclear single copy regions in Rosa canina genome assembly based on single copy orthologue (SCO) tags (Debray et al. 2019). We used 24 diploid rose species, pollen data from sekt. Caninae roses, Rosa canina subgenome specific SCO sequences, and outgroup species fr...
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Zusammenfassung: | This dataset was created to analyze nuclear single copy regions in Rosa
canina genome assembly based on single copy orthologue (SCO) tags (Debray
et al. 2019). We used 24 diploid rose species, pollen data from sekt.
Caninae roses, Rosa canina subgenome specific SCO sequences, and outgroup
species from the Rosaceae family. Target capturing baits were designed by
Agilent Technologies covered exons, UTRs, and small introns. The raw reads
of target capturer sequencing were processed with GATK to obtain a sample
specific reference for each SCO locus and each sample. Additionally,
single copy loci for all whole genome assemblies (Rosa canina, Rubus
idaeus, and Fragaria species) were extracted and concatenated into
subgenome-specific sequences and together with sample specific SCO
references used for alignment analysis. |
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DOI: | 10.5061/dryad.cc2fqz6fh |