Data from: Chimeric antigen receptors that trigger phagocytosis

Chimeric antigen receptors (CARs) are synthetic receptors that reprogram T cells to kill cancer. The success of CAR-T cell therapies highlights the promise of programmed immunity and suggests that applying CAR strategies to other immune cell lineages may be beneficial. Here, we engineered a family o...

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Hauptverfasser: Morrissey, Meghan A., Williamson, Adam P., Steinbach, Adriana M., Roberts, Edward W., Kern, Nadja, Headley, Mark B., Vale, Ronald, Vale, Ronald D
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creator Morrissey, Meghan A.
Williamson, Adam P.
Steinbach, Adriana M.
Roberts, Edward W.
Kern, Nadja
Headley, Mark B.
Vale, Ronald
Vale, Ronald D
description Chimeric antigen receptors (CARs) are synthetic receptors that reprogram T cells to kill cancer. The success of CAR-T cell therapies highlights the promise of programmed immunity and suggests that applying CAR strategies to other immune cell lineages may be beneficial. Here, we engineered a family of Chimeric Antigen Receptors for Phagocytosis (CAR-Ps) that direct macrophages to engulf specific targets, including cancer cells. CAR-Ps consist of an extracellular antibody fragment, which can be modified to direct CAR-P activity towards specific antigens. By screening a panel of engulfment receptor intracellular domains, we found that the cytosolic domains from Megf10 and FcRɣ robustly triggered engulfment independently of their native extracellular domain. We show that CAR-Ps drive specific engulfment of antigen-coated synthetic particles and whole human cancer cells. Addition of a tandem PI3K recruitment domain increased cancer cell engulfment. Finally, we show that CAR-P expressing murine macrophages reduce cancer cell number in co-culture by over 40%.
doi_str_mv 10.5061/dryad.c57c1s0
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identifier DOI: 10.5061/dryad.c57c1s0
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subjects Chimeric Antigen Receptor
Homo Sapiens
Mus musculus
Phagocytosis
title Data from: Chimeric antigen receptors that trigger phagocytosis
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