Resolving nerve fiber crossings at micrometer resolution in rat, vervet monkey, and human brain samples

Unstained histological sections obtained from a Wistar rat brain, two vervet monkey brains, and a human optic chiasm were measured with Scattered Light Imaging (SLI) to study nerve fiber crossings at micrometer resolution, e.g. in the corona radiata of the vervet monkey brain. The data provided here contains the original image stacks obtained from the SLI measurements as well as generated parameter maps showing different characteristics of the measured brain sections, e.g. the individual (in-plane) orientations for up to three (crossing) nerve fiber bundles for each measured image pixel (with a resolution down to 6.5 μm). For some of the measured brain sections, we also provide parameter maps generated from 3D-Polarized Light Imaging (3D-PLI) measurements for better comparison.