Supplemental Material for McClanahan et al., 2020
Supplemental Video File S1: Time lapse video of dehydration, rehydration, and recovery of C. elegans. A 10 µL droplet of N2 worms in M9 buffer containing 15% trehalose was pipetted on a PDMS surface and dehydrated for 12 h. The dehydrated droplet was transferred to a seeded NGM plate for recovery. S...
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Zusammenfassung: | Supplemental Video File S1: Time lapse video of dehydration, rehydration, and recovery of C. elegans. A 10 µL droplet of N2 worms in M9 buffer containing 15% trehalose was pipetted on a PDMS surface and dehydrated for 12 h. The dehydrated droplet was transferred to a seeded NGM plate for recovery. Supplemental Protocol File S2: Detailed protocol for dehydration, freezing, rehydration, and recovery of C. elegans. |
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DOI: | 10.25387/g3.13060790 |