Image: The SSU Processome Component Utp25p is a Pseudohelicase

Extended figure 2: The high pLDDT domains 1 and 2 of the AlphaFold yeast Utp25 align well to eIF4A. Structural alignment of the individual RecA-like domains 1 and 2 of the AlphaFold yeast Utp25 (P40498) and yeast eIF4A (1FUU; (Caruthers et al. 2000)) crystal structure. The AlphaFold yeast Utp25 is coloured based on pLDDT score, from very low confidence in red to very high confidence in blue (key in bottom right) whereas the yeast eIF4A crystal structure is shown in brown. In domain 1, six regions in the AlphaFold yeast Utp25 are coloured cyan (70 < pLDDT < 90; confident) as opposed to blue (pLDDT > 90, very high confidence). Two of these areas overlap with helicase motifs Q and Ib. Similarly, there are 7 confident areas in domain 2 (cyan), with one overlapping helicase motif VI. The remainder of the helicase motifs are in high confidence areas. The location of the helicase motifs is indicated along with the coordinates of the regions used in the structural alignments of isolated domains 1 and 2.