FNDC5/irisin Mediates the Protective Actions of Innovative Theta-shaking Exercise on Mouse Memory

T-Maze Test The T-maze test was analyzed using a chi-square test. The shaking group showed better accuracy and faster decision-making at 20 weeks. However, only the 30-week shaking group had a slight advantage (p=0.053). Passive Avoidance Task During memory acquisition, both groups behaved similarly. Memory regeneration showed improvement, with shaking group mice being less hesitant, indicating better memory retention. At 20 and 30 weeks, both groups improved memory, with the shaking group showing longer latency than the control. Histomorphology Effects FNDC5-Positive Reaction: FNDC5 was more pronounced in certain brain regions of the shaking group, including distal CA1, subiculum, DR MPFC, and VR MPFC. Abnormal Neurons: Shaking exercise resulted in fewer abnormal neurons in regions like upstream DG, proximal CA3, distal CA1, and DR MPFC. Number of Neurons: The subiculum subregion had more neurons in the shaking group. ELISA Effects Irisin: The shaking group had lower irisin levels in the wide-area brain and hippocampus. NGF Levels: The shaking group had higher NGF levels. NT4/5: No significant differences in NT4/5 levels between groups.