Evaluating the impact of TiO2nanoparticles with varying capping agents on cell integrity of Myroides odoratus isolated from spoilt paint coating

The impact of okro (O), basil (B) and spearmint (S) capped TiO2NPs on cell integrity of Myroides odoratus MZ442205 isolated from spoilt paint was investigated. TiO2NPs were synthesized through green routes. The antibacterial activity of the NPs at 5, 10, 20, 40 and 80 mg/ml was evaluated using diffusion technique. Damage on bacterium cell wall after NPs treatment was investigated using differential plating method, UV-Vis and SEM. The Zone of inhibition ranged from 2-5, 2-6, and1-3.5 mm; MIC was 20, 40 and 40 mg/ml while MBC was >80mg/ml for O, B and S capped TiO2NPs respectively. UV-Vis showed shifts in NPs compared to the bulk with absorptions at 211nm and 265nm respectively. Treated cells had 19-26% increase in absorbance at 260nm with increasing NPs concentration indicating leakages of nucleic acid materials into medium compared with the control without NPs. This was corroborated by reduced viable cell population in selective blood agar medium (8.15 x 104, 7.65 x 104 and 0.82 x 104CFU/mL) compared to general purpose nutrient broth (9.3 x 104, 8.7 x 104 and 0.88 x 104 CFU/mL) at 80mg/ml respectively. SEM showed spherical NPs, treated cells with cell wall pitting suggesting susceptibility to TiO2NPs via compromised cell integrity.