Comprehensive analysis of transcriptomics and metabolomics to reveal the mechanism of moxibustion ameliorating aging kidney injury

In the present study, we investigated the mechanism of moxibustion in improving renal injury in aging.Integrated analysis of untargeted metabolomics and transcriptomics revealed characteristic alterations in metabolites and genes in the aging kidney, and demonstrated that Cndp2, Pde7b, Pde1c, Adh7, and Adh1 were key targets of moxibustion in alleviating kidney injury of naturally aged mice, and the mechanism underlying this process could be achieved by regulating purine metabolism, histidine metabolism and glycolytic/gluconeogenic pathways. We presented four sets of data, all of which have been analyzed to identify differences among the three groups: young, old, and GS-Moxi. The first set includes kidney function indicators such as kidney organ coefficient, BUN and Scr data, as well as the evaluation of staining results Tubular injury score and Fibrotic area (%). The second set comprises heatmap, kegg pathway and ROC analysis of metabolomics. The third set consists of transcription omics data including heatmap and kegg pathway analysis. The fourth set contains validation data for gene expression and qRT-PCR in the transcriptomics of Cndp2, Pde7b, Pde1c, Adh7 and Adh1. All quantitative results were represented as the means±standard deviation (SD), and statistical analysis was performed by Graphpad Prism (Version 8.0). Differences between two groups were assessed using the unpaired two-tailed Student’s t-test. One-way analysis of variance was performed to compare more than two parametric groups. P