Pol II CTD droplet assay in different crowding reagent

Pol II CTD droplet assay in different crowding reagent

To screen the optimum condition for Pol II CTD droplet formation, the in different crowding reagent buffers were tested, including Mock, 10% dextran, 16% dextran, 10% glycerol, 1% PEG8000, 2.5 % PEG8000, 5 % PEG8000, and purified mEGFP and RUVBL1/2-mCherry proteins were used as controls. The best bu...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
1. Verfasser: Hui Wang
Format: Dataset
Sprache:eng
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:To screen the optimum condition for Pol II CTD droplet formation, the in different crowding reagent buffers were tested, including Mock, 10% dextran, 16% dextran, 10% glycerol, 1% PEG8000, 2.5 % PEG8000, 5 % PEG8000, and purified mEGFP and RUVBL1/2-mCherry proteins were used as controls. The best buffer for Pol II CTD droplet assay is 16% dextran in 20 mM Tris.HCl (pH7.5) and 150 mM NaCl.
DOI:10.17632/cxwv6dxdy5