SYNBIOCHEM Design-Build-Test-Learn pipeline

Quantified titers and peak values measured through different rounds of the SYNBIOCHEM Design-Build-Test-Learn (DBTL) pipeline for microbial production of fine chemicals. The pipeline was applied for the production of flavonoids and alkaloids in Escherichia coli. The flavonoids data contains cinnamate and (2S)-pinocembrin measurements for combinatorial libraries for: a) DBT round 1; b) DBT round 2; c) Chassis selection; d) Media screening; e) Optimization. The alkaloids data provides (S)-reticuline and (S)-scoulerine measurements for a combinatorial library corresponding to one DBTL round.