Effect of Mesenchymal Stem Cells With/Without Silymarin Against Liver Fibrosis in In Vitro Cell Line HepG2 with Study on Nuclear Factor-Kappa β, Caspase3, Apoptosis, Necrosis, Proliferation,and Collagen

We hypothesize Mesenchymal Stem Cells could Improve Liver Fibrosis. This research is an experimental pre and post test in vitro. Hepatocyte stem cells were taken from the umbilical cord of newborns, processed in the Prodia stem cell laboratory (Prostem). Hep.G2 cell line culture cells from ATCC, USA. Cell lines were divided into 4 groups: Cb (negative control), C1 (positive control / silymarin), C2 (Mesenchymal stem cells / MSC), C3 (MSC+Sylimarin), each group made 3 plates, consisting of 7 wells. Examination was carried out on NF-kβ, Caspase-3, Apoptosis, Necrosis, Proliferation, and cytopathologically seen the growth of collagen. Cytopathological test of cell line hepG2 showed fibroblast growth. In the isolation of UC-MSC, the phenotype results were CD 73+, CD90+, CD105+ and Lin- and condotioned medium BDNF, SDF1, FGF, VEGF, EGF, NGF, IL10, IL1β, IFN-α, MCP1, IL6, IL12p70, IL17A, IL18, IL23 NF-kβ test used Anova test, NF-kβ inhibition at 24 and 48 hours compared to control, significant p