Peptide Libraries from Cephalopods' Posterior Salivary Glands for Potential Antimicrobial Peptides

Filtered peptide libraries consisting of 11-40 amino acids (AA) were generated, comprising conventional AAs, and with intended use as a source of antimicrobial peptides (AMPs). These peptide libraries were constructed through various digestion protocols, including single enzyme applications (Trypsin, Chymotrypsin, Proteinase-K, AspN, and GluC), sequential mode with two enzymes (Tryp-Chym, Tryp-ProtK, Tryp-AspN, and Tryp-GluC), and concurrent mode with two enzymes (Tryp-Chym, Tryp-ProtK, Tryp-AspN, and Tryp-GluC). The peptide libraries were derived from the Composite Protein Database originating from Cephalopods' Posterior Salivary Glands.