A dataset on Ultra Sonication-Assisted Extraction of Natural Pigments from Dunaliella salina Microalgae using Synetude Reactor Apparatus

This study explores the sonication-assisted extraction of pigments from microalgae Dunaliella salina dry biomass using a Sonitube SM35, an ultrasonic reactor for continuous flow production (Syntude Chambery, France), with ethanol as solvent. The experiments were conducted at constant (35kHz) frequency with varying power (200W, 320W and 400W). The amplitudes of 100%, 80%, and 50% corresponding to powers of 400W, 320W, and 200W respectively were employed during sonication. The process was facilitated by a peristaltic pump-assisted flow of solvent-biomass mixture at a rate of 1000 ml/min. Dunaliella salina biomass (3 g) was dispersed in 500 ml ethanol and subjected to sonication for 30 min at 400, 320, and 200 W power. Samples were collected at five-minute intervals and aliquots were subsequently filtered prior to HPLC analysis. The experimental setup comprised two treatment groups: a control without an ice bath and an experimental treatment utilizing an ice bath to minimize heat generation during ultra-sonication. A separate set of control samples was maintained in the laboratory at 35°C under continuous shaking without sonication to serve as a comparative baseline. The samples were collected every 5 min interval and analyzed using high performance liquid chromatography (HPLC). The dataset provides the extraction efficiency of pigments viz., carotene, lutein, zeaxanthin and chlorophyll.