Eukaryotic microbial community at the LTER site Helgoland Roads from March 2016 to March 2019

Eukaryotic microbial community at the LTER site Helgoland Roads from March 2016 to March 2019

Water samples were collected from the long-term ecological research (LTER) site at Helgoland and the eukaryotic microbial community was assessed. 18S V4 region was amplified using the primer set 528iF /964iR and amplicon sequencing was performed on an Illumina MiSeq™ sequencer in a 2 × 300 bp paired...

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Hauptverfasser: Käse, Laura, Kraberg, Alexandra Claudia, Metfies, Katja, Neuhaus, Stefan, Sprong, Pim, Fuchs, Bernhard M, Boersma, Maarten, Wiltshire, Karen Helen
Format: Dataset
Sprache:eng
Schlagworte:
Binary Object
DNA barcoding
eukaryotic plankton
OTU
Phytoplankton
protists
Zooplankton
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Zusammenfassung:Water samples were collected from the long-term ecological research (LTER) site at Helgoland and the eukaryotic microbial community was assessed. 18S V4 region was amplified using the primer set 528iF /964iR and amplicon sequencing was performed on an Illumina MiSeq™ sequencer in a 2 × 300 bp paired-end run. Sequence data have been deposited in the European Nucleotide Archive (ENA) at the European Bioinformatics Institute (EMBL-EBI) under accession number PRJEB37135 using the data brokerage service of the German Federation for Biological Data (GFBio). 21 million sequences remained after bioinformatic processing and were clustered into Operational Taxonomic Units (OTUs). The Protist Ribosomal Reference database (PR2), version 4.11.1 was used as reference database.
DOI:10.1594/pangaea.921026