Sialidase assay and analysis of free sialic acid in tsetse (Glossina sp.) collected in protected and tsetse invested areas in Nigeria

This data set includes results of a sialidase assay applied in tsetse gut tissue from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. Each tsetse was identified morphologically and dissected in the field, all tissues preserved and used for further analysis. The whole gut of each tsetse was homogenized in the field and partially preserved for DNA analysis and used to investigate for sialidase activity with a fluorescent substrate and a natural substrate. Analysis for sialidase activity was done by fluorescent measurement and reverse phase HPLC of processed gut samples.