Recombinant fibronectin fragment III8-10/polylactic acid hybrid nanofibers enhance the bioactivity of titanium surface

To develop a nanofiber (NF)-based biomimetic coating on titanium (Ti) that mimics the complex spatiotemporal organization of the extracellular matrix (ECM). Recombinant cell attachment site (CAS) of fibronectin type III8-10 domain was co-electrospun with polylactic acid (PLA) and covalently bound on...

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Veröffentlicht in:	Nanomedicine (London, England) England), 2018-04, Vol.13 (8), p.899-912
Hauptverfasser:	Guillem-Marti, Jordi, Boix-Lemonche, Gerard, Gugutkov, Dencho, Ginebra, Maria-Pau, Altankov, George, Manero, Jose M
Format:	Artikel
Sprache:	eng
Schlagworte:	Adhesives Biological activity Biomedical materials Cell adhesion & migration Cell Adhesion - drug effects Cell Differentiation - drug effects Cell Line Cell Proliferation - drug effects electrospinning Enginyeria dels materials Ethanol fibronectin Fibronectins - chemistry Fibronectins - pharmacology Gene expression Humans hybrid nanofibers Mechanical properties Nanofibers - chemistry Nanofibres osseointegration Osseointegration - drug effects Osteoblasts - cytology Osteoblasts - drug effects Peptides PLA Polyesters - chemistry Polyesters - pharmacology Polylactic acid Polymers Prostheses and Implants Proteins recombinant protein Recombinant Proteins - chemistry Recombinant Proteins - pharmacology Surface Properties Tissue engineering Titanium Titanium - chemistry Titanium - pharmacology Transplants & implants Àrees temàtiques de la UPC
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Beschreibung
Zusammenfassung:	To develop a nanofiber (NF)-based biomimetic coating on titanium (Ti) that mimics the complex spatiotemporal organization of the extracellular matrix (ECM). Recombinant cell attachment site (CAS) of fibronectin type III8-10 domain was co-electrospun with polylactic acid (PLA) and covalently bound on polished Ti discs. Osteoblast-like SaOS-2 cells were used to evaluate their complex bioactivity. A significant increase of cell spreading was found on CAS/PLA hybrid NFs, followed by control pure PLA NFs and bare Ti discs. Cell proliferation showed similar trend being about twice higher on CAS/PLA NFs. The significantly increased ALP activity at day 21 indicated an enhanced differentiation of SaOS-2 cells. Coating of Ti implants with hybrid CAS/PLA NFs may improve significantly their osseointegration potential.
ISSN:	1743-5889 1748-6963
DOI:	10.2217/nnm-2017-0342