An unidentified cluster of infection in the Peruvian Amazon region
Bartonella bacilliformis is the etiological agent of Carrion's disease, which is a neglected disease linked to people in low-socioeconomic populations in Andean valleys. An outbreak of B. bacilliformis was reported in a rural area of the Peruvian Amazon region. The aim of this study was to char...
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Veröffentlicht in: | Journal of infection in developing countries 2015-05, Vol.9 (5), p.524-529 |
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container_title | Journal of infection in developing countries |
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creator | Cornejo, Angela Gomes, Claudia Suarez, Luis Martinez-Puchol, Sandra Bustamante, Pershing Pons, Maria J Ruiz, Joaquim Del Valle Mendoza, Juana |
description | Bartonella bacilliformis is the etiological agent of Carrion's disease, which is a neglected disease linked to people in low-socioeconomic populations in Andean valleys. An outbreak of B. bacilliformis was reported in a rural area of the Peruvian Amazon region. The aim of this study was to characterize this outbreak using molecular techniques.
Fifty-three blood samples from patients diagnosed with Carrion's disease were analyzed by molecular tools, using both a Bartonella-specific polymerase chain reaction (PCR) and an universal PCR, both based on 16S rRNA gene amplification. Additional water samples from the area were also analyzed.
Unexpectedly, the samples were positive only when the universal PCR was used. Although environmental contamination cannot be ruled out, the results showed that Sphingomonas faeni was the possible causative agent of this outbreak, and that water was the most feasible infection source.
Diagnosis by clinical criteria or microscopy may lead to misdiagnosis. There is a need to include molecular tools in the routine diagnosis of febrile syndromes, including Carrion's disease. |
doi_str_mv | 10.3855/jidc.6235 |
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Fifty-three blood samples from patients diagnosed with Carrion's disease were analyzed by molecular tools, using both a Bartonella-specific polymerase chain reaction (PCR) and an universal PCR, both based on 16S rRNA gene amplification. Additional water samples from the area were also analyzed.
Unexpectedly, the samples were positive only when the universal PCR was used. Although environmental contamination cannot be ruled out, the results showed that Sphingomonas faeni was the possible causative agent of this outbreak, and that water was the most feasible infection source.
Diagnosis by clinical criteria or microscopy may lead to misdiagnosis. There is a need to include molecular tools in the routine diagnosis of febrile syndromes, including Carrion's disease.</description><identifier>ISSN: 1972-2680</identifier><identifier>ISSN: 2036-6590</identifier><identifier>EISSN: 1972-2680</identifier><identifier>DOI: 10.3855/jidc.6235</identifier><identifier>PMID: 25989173</identifier><language>eng</language><publisher>Italy: Journal of Infection in Developing Countries</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Aigua ; Bacteris patògens ; Blood - microbiology ; Child ; Child, Preschool ; Disease Outbreaks ; DNA, Bacterial - genetics ; DNA, Ribosomal - genetics ; Female ; Gene amplification ; Gram-Negative Bacterial Infections - diagnosis ; Gram-Negative Bacterial Infections - epidemiology ; Gram-Negative Bacterial Infections - microbiology ; Humans ; Infant ; Male ; Middle Aged ; Parasites ; Paràsits ; Pathogenic bacteria ; Peru ; Peru - epidemiology ; Perú ; Polymerase Chain Reaction ; Reacció en cadena de la polimerasa ; RNA, Ribosomal, 16S - genetics ; Rural Population ; Sphingomonas - classification ; Sphingomonas - genetics ; Sphingomonas - isolation & purification ; Water ; Water Microbiology ; Young Adult</subject><ispartof>Journal of infection in developing countries, 2015-05, Vol.9 (5), p.524-529</ispartof><rights>2015. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>cc by (c) Cornejo et al., 2015 info:eu-repo/semantics/openAccess <a href="http://creativecommons.org/licenses/by/3.0/es/">http://creativecommons.org/licenses/by/3.0/es/</a></rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-ee52865586cc6076df022ee8b515d5e1c8453505d481f3237e63efc977d7efc73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,26972,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25989173$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cornejo, Angela</creatorcontrib><creatorcontrib>Gomes, Claudia</creatorcontrib><creatorcontrib>Suarez, Luis</creatorcontrib><creatorcontrib>Martinez-Puchol, Sandra</creatorcontrib><creatorcontrib>Bustamante, Pershing</creatorcontrib><creatorcontrib>Pons, Maria J</creatorcontrib><creatorcontrib>Ruiz, Joaquim</creatorcontrib><creatorcontrib>Del Valle Mendoza, Juana</creatorcontrib><title>An unidentified cluster of infection in the Peruvian Amazon region</title><title>Journal of infection in developing countries</title><addtitle>J Infect Dev Ctries</addtitle><description>Bartonella bacilliformis is the etiological agent of Carrion's disease, which is a neglected disease linked to people in low-socioeconomic populations in Andean valleys. An outbreak of B. bacilliformis was reported in a rural area of the Peruvian Amazon region. The aim of this study was to characterize this outbreak using molecular techniques.
Fifty-three blood samples from patients diagnosed with Carrion's disease were analyzed by molecular tools, using both a Bartonella-specific polymerase chain reaction (PCR) and an universal PCR, both based on 16S rRNA gene amplification. Additional water samples from the area were also analyzed.
Unexpectedly, the samples were positive only when the universal PCR was used. Although environmental contamination cannot be ruled out, the results showed that Sphingomonas faeni was the possible causative agent of this outbreak, and that water was the most feasible infection source.
Diagnosis by clinical criteria or microscopy may lead to misdiagnosis. There is a need to include molecular tools in the routine diagnosis of febrile syndromes, including Carrion's disease.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Aigua</subject><subject>Bacteris patògens</subject><subject>Blood - microbiology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Disease Outbreaks</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Female</subject><subject>Gene amplification</subject><subject>Gram-Negative Bacterial Infections - diagnosis</subject><subject>Gram-Negative Bacterial Infections - epidemiology</subject><subject>Gram-Negative Bacterial Infections - microbiology</subject><subject>Humans</subject><subject>Infant</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Parasites</subject><subject>Paràsits</subject><subject>Pathogenic bacteria</subject><subject>Peru</subject><subject>Peru - epidemiology</subject><subject>Perú</subject><subject>Polymerase Chain Reaction</subject><subject>Reacció en cadena de la polimerasa</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Rural Population</subject><subject>Sphingomonas - classification</subject><subject>Sphingomonas - genetics</subject><subject>Sphingomonas - isolation & purification</subject><subject>Water</subject><subject>Water Microbiology</subject><subject>Young Adult</subject><issn>1972-2680</issn><issn>2036-6590</issn><issn>1972-2680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>XX2</sourceid><recordid>eNpdkclOwzAQhi0EoqVw4AVQJC5wCHjJ2M6xVGxSJTjA2UqdCbhKk2InSPD0OGpZxMGaxd_8sucn5JjRC6EBLpeutBeSC9ghY5YrnnKp6e6ffEQOQlhSCrkAtk9GHHKdMyXG5GraJH3jSmw6VzksE1v3oUOftFXimgpt59omZkn3iskj-v7dFU0yXRWfse3xJd4ekr2qqAMebeOEPN9cP83u0vnD7f1sOk-tyGmXIgLXEkBLayVVsqwo54h6AQxKQGZ1BgIolJlmleBCoRRY2VypUsWoxISwja4NvTUeLXpbdKYt3G8xHE4VNxwUcBlnzjYza9--9Rg6s3LBYl0XDbZ9MExqnvFMUxrR03_osu19E38UxSRlnIHMI3W-fYRvQ_BYmbV3q8J_GEbNYIYZzDCDGZE92Sr2ixWWP-T39sUX7t6C-A</recordid><startdate>20150518</startdate><enddate>20150518</enddate><creator>Cornejo, Angela</creator><creator>Gomes, Claudia</creator><creator>Suarez, Luis</creator><creator>Martinez-Puchol, Sandra</creator><creator>Bustamante, Pershing</creator><creator>Pons, Maria J</creator><creator>Ruiz, Joaquim</creator><creator>Del Valle Mendoza, Juana</creator><general>Journal of Infection in Developing Countries</general><general>The Journal of Infection in Developing Countries</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8C1</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>XX2</scope></search><sort><creationdate>20150518</creationdate><title>An unidentified cluster of infection in the Peruvian Amazon region</title><author>Cornejo, Angela ; Gomes, Claudia ; Suarez, Luis ; Martinez-Puchol, Sandra ; Bustamante, Pershing ; Pons, Maria J ; Ruiz, Joaquim ; Del Valle Mendoza, Juana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-ee52865586cc6076df022ee8b515d5e1c8453505d481f3237e63efc977d7efc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Aigua</topic><topic>Bacteris patògens</topic><topic>Blood - microbiology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Disease Outbreaks</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Female</topic><topic>Gene amplification</topic><topic>Gram-Negative Bacterial Infections - diagnosis</topic><topic>Gram-Negative Bacterial Infections - epidemiology</topic><topic>Gram-Negative Bacterial Infections - microbiology</topic><topic>Humans</topic><topic>Infant</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Parasites</topic><topic>Paràsits</topic><topic>Pathogenic bacteria</topic><topic>Peru</topic><topic>Peru - epidemiology</topic><topic>Perú</topic><topic>Polymerase Chain Reaction</topic><topic>Reacció en cadena de la polimerasa</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Rural Population</topic><topic>Sphingomonas - classification</topic><topic>Sphingomonas - genetics</topic><topic>Sphingomonas - isolation & purification</topic><topic>Water</topic><topic>Water Microbiology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cornejo, Angela</creatorcontrib><creatorcontrib>Gomes, Claudia</creatorcontrib><creatorcontrib>Suarez, Luis</creatorcontrib><creatorcontrib>Martinez-Puchol, Sandra</creatorcontrib><creatorcontrib>Bustamante, Pershing</creatorcontrib><creatorcontrib>Pons, Maria J</creatorcontrib><creatorcontrib>Ruiz, Joaquim</creatorcontrib><creatorcontrib>Del Valle Mendoza, Juana</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Public Health Database</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Recercat</collection><jtitle>Journal of infection in developing countries</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cornejo, Angela</au><au>Gomes, Claudia</au><au>Suarez, Luis</au><au>Martinez-Puchol, Sandra</au><au>Bustamante, Pershing</au><au>Pons, Maria J</au><au>Ruiz, Joaquim</au><au>Del Valle Mendoza, Juana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An unidentified cluster of infection in the Peruvian Amazon region</atitle><jtitle>Journal of infection in developing countries</jtitle><addtitle>J Infect Dev Ctries</addtitle><date>2015-05-18</date><risdate>2015</risdate><volume>9</volume><issue>5</issue><spage>524</spage><epage>529</epage><pages>524-529</pages><issn>1972-2680</issn><issn>2036-6590</issn><eissn>1972-2680</eissn><abstract>Bartonella bacilliformis is the etiological agent of Carrion's disease, which is a neglected disease linked to people in low-socioeconomic populations in Andean valleys. An outbreak of B. bacilliformis was reported in a rural area of the Peruvian Amazon region. The aim of this study was to characterize this outbreak using molecular techniques.
Fifty-three blood samples from patients diagnosed with Carrion's disease were analyzed by molecular tools, using both a Bartonella-specific polymerase chain reaction (PCR) and an universal PCR, both based on 16S rRNA gene amplification. Additional water samples from the area were also analyzed.
Unexpectedly, the samples were positive only when the universal PCR was used. Although environmental contamination cannot be ruled out, the results showed that Sphingomonas faeni was the possible causative agent of this outbreak, and that water was the most feasible infection source.
Diagnosis by clinical criteria or microscopy may lead to misdiagnosis. There is a need to include molecular tools in the routine diagnosis of febrile syndromes, including Carrion's disease.</abstract><cop>Italy</cop><pub>Journal of Infection in Developing Countries</pub><pmid>25989173</pmid><doi>10.3855/jidc.6235</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Recercat |
subjects | Adolescent Adult Aged Aged, 80 and over Aigua Bacteris patògens Blood - microbiology Child Child, Preschool Disease Outbreaks DNA, Bacterial - genetics DNA, Ribosomal - genetics Female Gene amplification Gram-Negative Bacterial Infections - diagnosis Gram-Negative Bacterial Infections - epidemiology Gram-Negative Bacterial Infections - microbiology Humans Infant Male Middle Aged Parasites Paràsits Pathogenic bacteria Peru Peru - epidemiology Perú Polymerase Chain Reaction Reacció en cadena de la polimerasa RNA, Ribosomal, 16S - genetics Rural Population Sphingomonas - classification Sphingomonas - genetics Sphingomonas - isolation & purification Water Water Microbiology Young Adult |
title | An unidentified cluster of infection in the Peruvian Amazon region |
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