OmpA and Bap Genes as Virulence Genes Involved in Biofilm Formation of Acinetobacter baumannii
Background: Multiple virulence genes involved in biofilm development by Acinetobacter Baumannii (A. baumannii) like ompA and bap genes. These genes may be analyzed to establish their relevance in biofilm formation or antibiotic resistance phenotypes. This research objects to study virulence genes in...
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| Veröffentlicht in: | Journal of Advances in Medicine and Medical Research 2022-08, p.444-459 |
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| container_title | Journal of Advances in Medicine and Medical Research |
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| creator | Oraiby, Amira El Sayed El Bassiony Mohamed, Wesam Salah Elbaradey, Ghada Fouad Elenain, Amany Mohamed Abou Badreldein, Nagwa Mansour |
| description | Background: Multiple virulence genes involved in biofilm development by Acinetobacter Baumannii (A. baumannii) like ompA and bap genes. These genes may be analyzed to establish their relevance in biofilm formation or antibiotic resistance phenotypes. This research objects to study virulence genes involved in biofilm formation of A. baumannii to be taken in consideration in prophylactic approaches, treatment, and infection control program.
Methods: The prospective study contained 100 isolates including (sputum, endotracheal aspirate, pus from open wounds or open abscesses, mid-stream urine from non-catheterized subjects and catheter-stream urine in catheterized subjects, blood samples and body fluids) were collected from different ICUs. The BD Phoenix Automated System was used to identify the isolates and assess their susceptibility to 21 antibiotics. Biofilm formation was measured by microtiter plate for all isolates, then conventional PCR was done for detection of ompA and bap genes.
Results: The comparison of biofilm strength and MDR revealed significant variance among different groups (P |
| doi_str_mv | 10.9734/jammr/2022/v34i2131563 |
| format | Article |
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Methods: The prospective study contained 100 isolates including (sputum, endotracheal aspirate, pus from open wounds or open abscesses, mid-stream urine from non-catheterized subjects and catheter-stream urine in catheterized subjects, blood samples and body fluids) were collected from different ICUs. The BD Phoenix Automated System was used to identify the isolates and assess their susceptibility to 21 antibiotics. Biofilm formation was measured by microtiter plate for all isolates, then conventional PCR was done for detection of ompA and bap genes.
Results: The comparison of biofilm strength and MDR revealed significant variance among different groups (P<0.05). The presence or absence of OmpA and bap genes was associated with biofilm biomass (with a P value <0.001). Among the 100 A. baumannii isolates, both genes were found in 44 isolates (44%). The strains having both OmpA and Bap genes (27/27) produce stronger biofilms (100%) than those with just one gene only one gene only (P <0.001). The association among the presence of virulence genes and MDR status was measured. The genes encoding OmpA were found at a higher frequency in MDRAB than in non-MDRAB strains (P=0.014). Bap gene was found in 48/90 (53.3%) MDRAB isolates versus only 2/10 (20%) of non-MDRAB strains (P=0.046).
Conclusions: There is a significant association among MDR and the biofilm-forming ability of A. baumannii. Biofilm-related genes (ompA and bap) are connected with multidrug-resistant A. baumannii strains and affect the intensity of biofilm formation.</description><identifier>ISSN: 2456-8899</identifier><identifier>EISSN: 2456-8899</identifier><identifier>DOI: 10.9734/jammr/2022/v34i2131563</identifier><language>eng</language><ispartof>Journal of Advances in Medicine and Medical Research, 2022-08, p.444-459</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Oraiby, Amira El Sayed El Bassiony</creatorcontrib><creatorcontrib>Mohamed, Wesam Salah</creatorcontrib><creatorcontrib>Elbaradey, Ghada Fouad</creatorcontrib><creatorcontrib>Elenain, Amany Mohamed Abou</creatorcontrib><creatorcontrib>Badreldein, Nagwa Mansour</creatorcontrib><title>OmpA and Bap Genes as Virulence Genes Involved in Biofilm Formation of Acinetobacter baumannii</title><title>Journal of Advances in Medicine and Medical Research</title><description>Background: Multiple virulence genes involved in biofilm development by Acinetobacter Baumannii (A. baumannii) like ompA and bap genes. These genes may be analyzed to establish their relevance in biofilm formation or antibiotic resistance phenotypes. This research objects to study virulence genes involved in biofilm formation of A. baumannii to be taken in consideration in prophylactic approaches, treatment, and infection control program.
Methods: The prospective study contained 100 isolates including (sputum, endotracheal aspirate, pus from open wounds or open abscesses, mid-stream urine from non-catheterized subjects and catheter-stream urine in catheterized subjects, blood samples and body fluids) were collected from different ICUs. The BD Phoenix Automated System was used to identify the isolates and assess their susceptibility to 21 antibiotics. Biofilm formation was measured by microtiter plate for all isolates, then conventional PCR was done for detection of ompA and bap genes.
Results: The comparison of biofilm strength and MDR revealed significant variance among different groups (P<0.05). The presence or absence of OmpA and bap genes was associated with biofilm biomass (with a P value <0.001). Among the 100 A. baumannii isolates, both genes were found in 44 isolates (44%). The strains having both OmpA and Bap genes (27/27) produce stronger biofilms (100%) than those with just one gene only one gene only (P <0.001). The association among the presence of virulence genes and MDR status was measured. The genes encoding OmpA were found at a higher frequency in MDRAB than in non-MDRAB strains (P=0.014). Bap gene was found in 48/90 (53.3%) MDRAB isolates versus only 2/10 (20%) of non-MDRAB strains (P=0.046).
Conclusions: There is a significant association among MDR and the biofilm-forming ability of A. baumannii. Biofilm-related genes (ompA and bap) are connected with multidrug-resistant A. baumannii strains and affect the intensity of biofilm formation.</description><issn>2456-8899</issn><issn>2456-8899</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqdz81Kw0AUBeBBFCzaV5D7AjWZn6bNspVWXbkRlw436Q1cydwJM2nAty-VLrp2dQ4HzuJT6kmXz_XKuuIHQ0iFKY0pJuvYaKuXlb1RM-OW1WK9ruvbq36v5jlzUzq3ctZYPVPfH2HYAMoBtjjAKwllwAxfnI49SUuX6V2m2E90ABbYcuy4D7CPKeDIUSB2sGlZaIwNtiMlaPAYUIT5Ud112GeaX_JBVfvd58vbok0x50SdHxIHTL9el_4M8n8gfwb5K5D99_EEOztYiA</recordid><startdate>20220830</startdate><enddate>20220830</enddate><creator>Oraiby, Amira El Sayed El Bassiony</creator><creator>Mohamed, Wesam Salah</creator><creator>Elbaradey, Ghada Fouad</creator><creator>Elenain, Amany Mohamed Abou</creator><creator>Badreldein, Nagwa Mansour</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20220830</creationdate><title>OmpA and Bap Genes as Virulence Genes Involved in Biofilm Formation of Acinetobacter baumannii</title><author>Oraiby, Amira El Sayed El Bassiony ; Mohamed, Wesam Salah ; Elbaradey, Ghada Fouad ; Elenain, Amany Mohamed Abou ; Badreldein, Nagwa Mansour</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-crossref_primary_10_9734_jammr_2022_v34i21315633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Oraiby, Amira El Sayed El Bassiony</creatorcontrib><creatorcontrib>Mohamed, Wesam Salah</creatorcontrib><creatorcontrib>Elbaradey, Ghada Fouad</creatorcontrib><creatorcontrib>Elenain, Amany Mohamed Abou</creatorcontrib><creatorcontrib>Badreldein, Nagwa Mansour</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of Advances in Medicine and Medical Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oraiby, Amira El Sayed El Bassiony</au><au>Mohamed, Wesam Salah</au><au>Elbaradey, Ghada Fouad</au><au>Elenain, Amany Mohamed Abou</au><au>Badreldein, Nagwa Mansour</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>OmpA and Bap Genes as Virulence Genes Involved in Biofilm Formation of Acinetobacter baumannii</atitle><jtitle>Journal of Advances in Medicine and Medical Research</jtitle><date>2022-08-30</date><risdate>2022</risdate><spage>444</spage><epage>459</epage><pages>444-459</pages><issn>2456-8899</issn><eissn>2456-8899</eissn><abstract>Background: Multiple virulence genes involved in biofilm development by Acinetobacter Baumannii (A. baumannii) like ompA and bap genes. These genes may be analyzed to establish their relevance in biofilm formation or antibiotic resistance phenotypes. This research objects to study virulence genes involved in biofilm formation of A. baumannii to be taken in consideration in prophylactic approaches, treatment, and infection control program.
Methods: The prospective study contained 100 isolates including (sputum, endotracheal aspirate, pus from open wounds or open abscesses, mid-stream urine from non-catheterized subjects and catheter-stream urine in catheterized subjects, blood samples and body fluids) were collected from different ICUs. The BD Phoenix Automated System was used to identify the isolates and assess their susceptibility to 21 antibiotics. Biofilm formation was measured by microtiter plate for all isolates, then conventional PCR was done for detection of ompA and bap genes.
Results: The comparison of biofilm strength and MDR revealed significant variance among different groups (P<0.05). The presence or absence of OmpA and bap genes was associated with biofilm biomass (with a P value <0.001). Among the 100 A. baumannii isolates, both genes were found in 44 isolates (44%). The strains having both OmpA and Bap genes (27/27) produce stronger biofilms (100%) than those with just one gene only one gene only (P <0.001). The association among the presence of virulence genes and MDR status was measured. The genes encoding OmpA were found at a higher frequency in MDRAB than in non-MDRAB strains (P=0.014). Bap gene was found in 48/90 (53.3%) MDRAB isolates versus only 2/10 (20%) of non-MDRAB strains (P=0.046).
Conclusions: There is a significant association among MDR and the biofilm-forming ability of A. baumannii. Biofilm-related genes (ompA and bap) are connected with multidrug-resistant A. baumannii strains and affect the intensity of biofilm formation.</abstract><doi>10.9734/jammr/2022/v34i2131563</doi></addata></record> |
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| source | Alma/SFX Local Collection |
| title | OmpA and Bap Genes as Virulence Genes Involved in Biofilm Formation of Acinetobacter baumannii |
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